Titration of the His Tag antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000
Restrictions
For Research Use only
Format
Liquid
Buffer
In 1X PBS, pH 7.4, with 0.09 % sodium azide
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
-20 °C
Stockage commentaire
Aliquot the His Tag antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Antigène
His Tag
Classe de substances
Tag
Sujet
Epitope tags consisting of short sequences recognized by well-characterized monoclonal or polyclonal antibodies have been widely used in the study of protein expression in various systems. The 6xHIS tag (HHHHHH), recognized by the monoclonal or polyclonal antibody provides an established example of this application. 6xHIS-tagged fusion proteins are easily purified by affinity chromatography using Nickel-Sepharose resin. NSJBio's anti-6xHIS polyclonal antibody provides a simple solution to detect the expression of HIS-tagged fusion proteins.